Mycelial Growth of Pleurotus spp. Isolates in the Light and the Dark

Authors

  • Tainara Reis Santos Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Laboratório de Microbiologia Agrícola, Campus de São Cristóvão. SE, Brazil. https://orcid.org/0009-0001-1672-448X
  • Ester Da Silva Murta Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Laboratório de Microbiologia Agrícola, Campus de São Cristóvão. SE, Brazil. https://orcid.org/0009-0008-1822-0470
  • Andréa Verônica Gobbi Barbosa Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Laboratório de Microbiologia Agrícola, Campus de São Cristóvão. SE, Brazil. https://orcid.org/0000-0002-3562-3457
  • Marcos Cabral de Vasconcellos Barretto Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Laboratório de Remediação de solos. SE, Brazil. https://orcid.org/0000-0002-2456-7541
  • Regina Helena Marino Universidade Federal de Sergipe, Departamento de Engenharia Agronômica, Laboratório de Microbiologia Agrícola, Campus de São Cristóvão. SE, Brazil. https://orcid.org/0000-0002-7295-3746

Abstract

The white rot fungus Pleurotus spp. traditionally cultivated on lignocellulolytic waste and used as  food for a healthy diet, has also been exploited in the production of biocomposites. However, little is known about the effect of light on the mycelial growth of Pleurotus spp. in the culture medium. The aim of this study was to evaluate the influence of room light and continuous darkness on the mycelial growth of Pleurotus spp. isolates in vitro. The design used was entirely randomized in a 6 x 2 x 3 factorial scheme, corresponding to the cultivation of six fungal isolates (Pleurotus ostreatus: DF39, EF39, EF60, POS AJU1 and POS W; and Pleurotus ostreatoroseus: POS SP1), in two lighting conditions (room  light and continuous dark) and three evaluation periods (3rd, 4th and 5th day after inoculation, with four replicates per treatment). For fungal isolate, luminosity did not influence mycelial diameter, growth rate and mycelial density. The increase in the cultivation period, regardless of light and fungal isolates, favored mycelial density. Compared to cultivation in room light, darkness favored an increase in mycelial diameter and mycelial growth rate, depending on the Pleurotus spp. isolate.

Keywords: Basidiomycetes. Luminosity. Oyster Mushroom.

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Published

2025-07-25

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REIS SANTOS, Tainara; DA SILVA MURTA, Ester; GOBBI BARBOSA, Andréa Verônica; CABRAL DE VASCONCELLOS BARRETTO, Marcos; MARINO, Regina Helena. Mycelial Growth of Pleurotus spp. Isolates in the Light and the Dark. Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde, [S. l.], v. 29, n. 3, p. 595–607, 2025. Disponível em: https://ensaioseciencia.pgsscogna.com.br/ensaioeciencia/article/view/13780. Acesso em: 27 jul. 2025.

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Vol. 29 No. 3 (2025)

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Copyright (c) 2025 Tainara Reis Santos, Ester Da Silva Murta, Andréa Verônica Gobbi Barbosa, Marcos Cabral de Vasconcellos Barretto, Regina Helena Marino

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