Antimicrobial Activity and Synergism Between Coix lacryma-jobi Extract and Three Antibiotics Against Pseudomonas aeruginosa
DOI:
https://doi.org/10.17921/1415-6938.2025v29n3p670-680Abstract
There is a wide variety of plants with active ingredients with antimicrobial activity. Coix lacryma-jobi, one of the species whose compounds have already been identified, is popularly known as Job's tear and has been used to treat various diseases, such as rheumatic diseases, neuralgia and female endocrine disorders, as it has anti-inflammatory, antitumor, antiallergic, and antimicrobial properties. The objective of this study was to evaluate the synergism of polar extract of Coix lacryma-jobi and antibiotics (ciprofloxacin, chloramphenicol and gentamicin) against the bacterium Pseudomonas aeruginosa. The material was collected in a vacant lot, it was dried in a forced air oven and ground in a blender and subjected to extraction in organic solvent ethyl acetate, over 28 days occasional agitations were made, then the bottle was reserved at room temperature for solvent evaporation. The extract was tested along with specific antibiotics for P. aeruginosa. It was concluded that the extract of the leaves of C. lacryma-jobi has an inhibitory effect against the strain of Pseudomonas aeruginosa tested. There was synergism between the leaf extract and gentamicin increasing its antimicrobial activity. It was also observed that the strain showed high tolerance to chloramphenicol, and that it inhibited the effect of C. lacryma-jobi extract. Further tests, especially in vivo, are suggested to evaluate the efficacy or otherwise of using the antibiotic gentamicin together with C. lacryma-jobi extract, and to better understand the antagonistic effect demonstrated to chloramphenicol.
Keywords: P. aeruginosa. Coix lacryma-jobi. Medicinal Plants.
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