Morphological and Molecular Identification of Yeasts Isolated from Brazilian Savannah Fruits Submitted to Concomitant Stress in Sugarcane Juice
Abstract
In a biotechnological system, the precise species identification is paramount for an efficient process. In industrial conditions, fermentation is conducted without aseptic, and consequently, the process is subject to constant contamination by bacteria and yeast strains, including wild species of Saccharomyces cerevisiae. The characterization and identification of yeast species have been based on morphology and physiological characteristics. However, these laborious techniques often leave room for doubt. Currently, molecular biology techniques allow the rapid identification of yeast species involved in the fermentation process efficiently and economically. The bioprospection of yeast more resistant to stress conditions may reveal strains with good potential for use. However, correct isolate molecular and morphological characterization is required. The present study aimed to characterize five yeasts isolated from Brazilian savannah fruit extracts on a morphological and molecular level. Morphological analysis of the yeasts L32, L34, L36, L38, and B32 considered factors such as colony size, color, surface, and edges. This characterization was complemented with molecular identification performed using the PCR and PCR/RFLP. The results show predominantly smooth and shiny colonies. Analysis of the amplification of ribosomal DNA fragments, after digestion with the enzymes Hae III and Hinf I, resulted in bands corresponding to the profile of S. cerevisiae. With these results, it was possible to conclude that the method of yeast isolation by pressure of simultaneous stresses allows the selection of the S. cerevisiae yeasts.
Keywords: Selective Isolation. PCR. PCR/RFLP. Ribosomal DNA. Colonies.
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Copyright (c) 2025 Regilene Fátima De Oliveira, Bruno Fines, Valéria Cristina Ferreira Da Silva, Marney Pascoli Cereda, Antonia Railda Roel, Carina Elisei De Oliveira
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