Multivariate Approach Helps in Understanding the Tritrophic Interaction of Bt cotton, Aphis gossypii and Chrysoperla externa
Abstract
In cotton, Chrysoperla externa stands out for its ability to prey on arthropods. The aim of this study was to evaluate the reproductive aspects and longevity of Chrysoperla externa when the larvae were fed Aphis gossypii from transgenic (Bt) and conventional cotton. Nine cotton cultivars were used: Conventional FM993, DP604 BG (Bollgard I®-Cry1Ac), NuOpal (Bollgard I®-Cry1Ac), DP90 B (Bollgard I®-Cry1Ac), Acala DTL90B (Bollgard I®-Cry1Ac), DP555 BGRR (Bollgard I®-Cry1Ac), DP1228 B2RF (Bollgard II®-Cry1Ac+Cry2Ab2), PHY440 W (WideStrike®-Cry1Ac+Cry1F) and FM975 WS (WideStrike®-Cry1Ac+Cry1F). The adults of C. externa from the F5 generation, in which the larvae were fed A. gossypii from the cotton cultivars, were sexed and kept as a pair per cage. Longevity and reproductive potential were assessed daily (preoviposition, oviposition and postoviposition periods; daily and total oviposition capacity; percentage of eggs without pedicels; and egg viability). Principal component (PC) and cluster analyses were performed using the all measured variables. All variables showed that there is variation in the response of C. externa fed during the immature stages with A. gossypii from cotton cultivars with different Bt technologies. C. externa larvae that fed on A. gossypii from transgenic plants presented a reduction in reproductive potential and adult longevity. The PC and the formed 3 groups for the CA analyses, mostred the reproductive aspects and longevity of C. externa were negatively influenced when the larvae were fed A. gossypii from the cultivars DP90 B and DP555 BGRR, which express the Cry1Ac protein and affect genetic characteristics when the larvae are fed the nontarget pest.
Keywords: Chrysopids. Cotton Aphid. Reproductive Potential. Transgenic Cotton.
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Copyright (c) 2025 Pamella Mingotti Dias, Luciana Claudia Toscano, Noemi Cristina De Souza Vieira, Jorge González Aguilera, Wilson Itamar Maruyama
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