Molecular Differentiation of Rural Tuberculosis Cases in Mato Grosso: Investigating the Rv2807 and TbD1 Genes by Nested q-PCR

Authors

  • Lauren Cristiane Leite Ocampos Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil. https://orcid.org/0009-0009-8543-4409
  • Andressa Ricci Biz Messias Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil. https://orcid.org/0000-0002-6304-9050
  • Doracilde Terumi Takahara Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.
  • Elaine Cristina de Oliveira Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.
  • Klaucia Rodrigues Vasconcelos Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.
  • Tony Edgley Catão Tenório Unopar, Programa de Pós-Graduação em Produção Animal. PR, Brazil.
  • Adelino Cunha Neto Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil. https://orcid.org/0000-0003-4365-9502
  • Ricardo César Tavares Carvalho Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil. https://orcid.org/0000-0002-0666-7135

DOI:

https://doi.org/10.17921/1415-6938.2026v30n2p332-340

Abstract

Among infectious diseases, human tuberculosis (TB) remains one of the major public health problems. Information regarding groups vulnerable to this disease caused by Mycobacterium bovis is scarce both nationally and internationally. Therefore, this research aimed to investigate the occurrence of M. bovis among patients with sputum positive for the Mycobacterium tuberculosis complex (TBMC), from rural areas of some cities in Mato Grosso. To this end, the target gene Rv2807 was evaluated using Nested q-PCR to confirm TBMC, and for M. bovis by detecting the TbD1 region, directly from DNA extracted from nine sputum samples with positive TBMC cultures provided by LACEN-MT, selected from 61 individuals diagnosed with pulmonary tuberculosis by LACEN/MT between 2017 and 2023. After heat inactivation using the Dneasy Blood & Tissue kit - Qiagen, DNA was extracted according to the manufacturer's instructions. The nine samples tested were confirmed as belonging to the Mycobacterium tuberculosis Complex through the presence of the Rv2807 gene. However, the TbD1 region was not observed, revealing the absence of Mycobacterium bovis among patients positive for the Mycobacterium tuberculosis Complex. Even so, this highlights the importance of investing in molecular testing for species belonging to the Mycobacterium genus in samples positive for both bovine and human tuberculosis. It should be emphasized that tuberculosis remains a serious cause of infectious health problems in Brazil, making it necessary to investigate the species detected in confirmed TB cases more precisely, especially in rural populations and agricultural workers.   Keywords: Mycobacterium bovis. Tuberculosis. Mycobacterium tuberculosis Complex. One Health.   Resumo Entre as doenças infecciosas a tuberculose humana (TB) permanece sendo um dos grandes problemas de saúde pública. E informações sobre grupos vulneráveis à esta patologia desencadeada pelo Mycobaterium bovis são poucas nacional e/ou internacionalmente. Portanto, esta pesquisa objetivou averiguar a ocorrência M. bovis entre pacientes com escarros positivos para o complexo Mycobacterium tuberculosis (CMTB), provenientes da zona rural de algumas cidades de Mato Grosso. Para tanto, se avaliou através do Nested q-PCR para confirmação do CMTB o gene alvo Rv2807, e para M. bovis pela detecção da região TbD1, diretamente do DNA extraído de nove amostras de escarro com cultura positiva para CMTB cedidos pelo LACEN-MT, selecionados entre 61 indivíduos diagnosticados com Tuberculose pulmonar pelo LACEN/MT no período de 2017 a 2023. Após a inativação pelo calor, e utilizando o kit Dneasy Blood & Tissue- Qiagen, extraiu-se o DNA. As nove amostras testadas foram confirmadas como pertencentes ao Complexo Mycobacterium tuberculosis, através da presença do gene Rv2807. No entanto, não se observou a presença da região TbD1, revelando a ausência de Mycobacterim bovis entre os pacientes positivos para o Complexo Mycobacterium tuberculosis. Mesmo assim se destaca aqui a importância no investimento de testagem molecular para espécies pertencentes ao gênero Mycobacterium diante de amostras positivas para tuberculose tanto bovina, quanto humana. Pode se ressaltar que a tuberculose continua uma causa grave de agravo a saúde de origem infecciosa no Brasil, sendo necessário a investigação mais precisa das espécies detectadas nos casos de TB confirmados, principalmente em população rural e trabalhadores rurais.   Palavras-chave: Complexo Mycobacterium tuberculosis. Mycobacterium bovis. Tuberculose. Saúde Única. 

 

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Author Biographies

  • Lauren Cristiane Leite Ocampos, Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil.

    Holds a degree in Nursing from the Federal University of Mato Grosso (1999). Employee of the State Health Secretariat of Mato Grosso. Has experience in the area of ​​Nursing, with emphasis on Cardiovascular Surgery and Health Surveillance/Evaluation in Health Services. Master's degree in Public Health (class of 2016/1) from the Federal University of Mato Grosso, Institute of Public Health, area of ​​expertise: Epidemiology. PhD in Animal Biosciences from UNIC (2024/2). Professor in the Medicine internship I and II in public health, Nursing course, and medical residency in family health at the University Center of Várzea Grande - UNIVAG.

  • Andressa Ricci Biz Messias, Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil.

    She holds a degree in Biological Sciences from the Federal University of Mato Grosso (2010), a master's degree in Tropical Agriculture from the Federal University of Mato Grosso (2014), and specializations in Biotechnology (2023) and Applied Statistics (2024). She is currently pursuing a teaching qualification in Higher Education (starting in 2024). She was an associate researcher I at UFMT and currently conducts research in the Molecular Biology and Microbiology laboratory at the University of Cuiabá. She has experience in the field of Microbiology, with an emphasis on Microbiology, mainly working on the following topics: molecular biology (currently with an emphasis on real-time PCR) and biotechnology. She is currently a doctoral student in Animal Bioscience at the University of Cuiabá, Mato Grosso.

  • Doracilde Terumi Takahara, Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.

    She holds a degree in Pharmaceutical Biochemistry from the State University of Maringá - PR (1984), a postgraduate degree in Clinical Analysis from the University of Cuiabá - MT (2006), and is a civil servant at the Mato Grosso State Health Secretariat (working at LACEN-MT since 2004). She holds a Master's degree in Health Sciences, specializing in Infectious and Tropical Diseases, from the Faculty of Medicine of the Federal University of Mato Grosso (2011). She worked at the UFMT Research Laboratory through a partnership established between the Mato Grosso State Health Secretariat and UFMT (Federal University of Mato Grosso), in the Mycology sector, performing serological tests for Paracoccidioidomycosis (2012-2017). She currently works in the Mycobacteriology sector, isolating Non-Tuberculous Mycobacteria and Mycobacterium tuberculosis in particular (2004-2022).

  • Elaine Cristina de Oliveira, Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.

    Graduated in Biological Sciences (2004) and Pedagogy, Specialist in Public Health, with emphasis on Local Health Systems - ESP/SES/MT, in Educational Processes in Health with emphasis on facilitation in Active Methodologies - Hospital Sirio Libanes - HSL (2014), in Educational Processes in Health with emphasis on Competency Assessment - Hospital Sirio Libanes - HSL (2017), in Continuing Education in Health - UFRGS (2016). Master's degree in Collective Health - Epidemiology - ISC/UFMT (2009) and PhD in Health Sciences - FCM/UFMT (2017). Coordinator of the State Malaria Control Program at the State Health Secretariat/Government of the State of Mato Grosso (2005 to 2018). Director of the Central Public Health Laboratory of Mato Grosso (since 02/2019). With experience in the areas of Public Health, Epidemiology, Research Methodology, Prevention and control of endemic diseases, Geoprocessing and Remote Sensing. Experience in public health emergencies and quality management.

  • Klaucia Rodrigues Vasconcelos, Laboratório Central de Saúde Pública do Estado de Mato Grosso, Departamento de Micobacteriologia. MT, Brazil.

    She holds a degree in Biomedicine from the University Center of Várzea Grande and in Clinical Cytology from the Arel Iuga Institute of Education. She has a specialization in Higher Education Teaching from the University Center of Várzea Grande and in Epidemiological Surveillance from the Faculdade Brasil Empreender (FABEMP). She also completed a Lato Sensu Postgraduate degree in Public Health from Faculdade UniBF. Currently, she works as Technical Coordinator and Technical Manager of the Central Public Health Laboratory of Mato Grosso (LACEN/MT).

  • Tony Edgley Catão Tenório, Unopar, Programa de Pós-Graduação em Produção Animal. PR, Brazil.

    Graduated in Veterinary Medicine from the Federal Rural University of Pernambuco (2001). Postgraduate degree in Animal Health Defense from Faculdade Santo André (2015). Postgraduate degree in Hygiene, Inspection and Technology of Products of Animal Origin from Faculdade de Tecnologia de Curitiba (2018). Bachelor's degree in Agribusiness Technology from Universidade Cesumar - Unicesumar (2021). Master's degree in Animal Production from Universidade Brasil (2024), currently pursuing a doctorate in Animal Production from Universidade do Norte do Paraná. State Agricultural Tax Auditor at the Rondônia State Agricultural and Livestock Defense Agency (IDARON) since May 18, 2004, working in the area of ​​Animal Health Defense and Inspection of Products of Animal Origin.

  • Adelino Cunha Neto, Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil.

    A nutritionist with a Ph.D. in Veterinary Medicine. He researches microbial contamination in food of animal and plant origin, including parasites, toxigenic fungi, and bacterial pathogens. He has experience in teaching and food safety diagnostics.

  • Ricardo César Tavares Carvalho, Universidade de Cuiabá, Programa de Pós-Graduação em Biociência Animal. MT, Brazil.

     Holds a Ph.D. in Food Science and is a professor at UNIC. He coordinates the graduate program in Animal Biosciences and investigates foodborne pathogens, antimicrobial resistance, and molecular microbiology in fish and animal-based foods

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Published

2026-09-21

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Vol. 30 No. 2 (2026)

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Articles

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Copyright (c) 2026 Lauren Cristiane Leite Ocampos, Andressa Ricci Biz Messias, Doracilde Terumi Takahara, Elaine Cristina de Oliveira, Klaucia Rodrigues Vasconcelos, Tony Edgley Catão Tenório, Adelino Cunha Neto, Ricardo César Tavares Carvalho

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How to Cite

Molecular Differentiation of Rural Tuberculosis Cases in Mato Grosso: Investigating the Rv2807 and TbD1 Genes by Nested q-PCR. Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde, [S. l.], v. 30, n. 2, p. 332–340, 2026. DOI: 10.17921/1415-6938.2026v30n2p332-340. Disponível em: https://ensaioseciencia.pgsscogna.com.br/ensaioeciencia/article/view/15262. Acesso em: 26 sep. 2026.